Contribution of Genome–environment Interaction to Pre-eclampsia in a Havana Maternity Hospital

INTRODUCTION Pre-eclampsia (PE) is a pregnancy-induced hypertensive disorder , accompanied by edema, proteinuria or both, occurring at >20 weeks gestation. It is primarily affects nulliparous women with no history of cardiovascular or kidney problems and is reversible post partum.[1] PE is a leading cause of morbidity and mortality in pregnancy worldwide, accounting for 3–8% of pregnancy complications, including perinatal death, preterm birth and intrauterine growth retardation.[1–5] Together with eclampsia, PE is responsible for 10–15% of the over 500,000 deaths from pregnancy-related disorders globally (99% of these in developing countries).[1,3] In Cuba, PE is among the leading causes of maternal death and there has been an apparent rise in the proportion of maternal mortality related to hypertensive disorders of pregnancy since 2009, but numbers are small: 4 maternal deaths in 2012, a rate of 3.2 per 100,000 live births.[6,7] The precise etiology of PE is unknown. However, it is considered a complex, multfactoral disease, in which individual manifestations depend on the interaction of two or more maternal genes with the fetal genotype and with environmental factors.[8] The main risk factors associated with PE and eclampsia are maternal age (risk is higher at the extremes of age, <18 or >35 years), nulliparity, malnutrition, poverty, low educational level, multiple pregnancy, molar pregnancy, diabetes and lupus erythematosus.[8] Other factors associated with elevated PE risk are s protein defi ciency, anticardiolipin antibodies, pre-pregnancy obesity, and genetic factors such as family history (mothers, sisters and daughters of PE patients have greater frequency of the disorder).[6,8] Some studies have identifi ed chromosome regions and candidate genes whose variants are related to greater PE susceptibility, but their results have not been replicated consistently across populations .[9,10] The following have been shown to increase PE susceptibility in different settings:[9] • polymorphism of the methylenetetrahydrofolate reductase enzyme gene, a fl avoprotein involved in homocysteine remeth-ylation; • a common mutation leading to substitution of a glutamine by an arginine at position 506 in the gene encoding for factor V Leiden; • angiotensinogen gene polymorphisms; • common polymorphisms in the gene encoding for lipoprotein lipase; and • the NOS3 gene, located at region 7q36, which encodes for endothelial nitric oxide synthase. The US National Center for Biotechnology Information genome database includes other genes that may predispose to PE: the TGF-a gene; the alpha-adducin gene, which encodes a protein associated with calmodulin, whose mutations generate changes in blood pressure regulation in rats; the …


INTRODUCTION
Pre-eclampsia (PE) is a pregnancy-induced hypertensive disorder, accompanied by edema, proteinuria or both, occurring at >20 weeks gestation.It is primarily affects nulliparous women with no history of cardiovascular or kidney problems and is reversible post partum.[1] PE is a leading cause of morbidity and mortality in pregnancy worldwide, accounting for 3 8% of pregnancy complications, including perinatal death, preterm birth and intrauterine growth retardation.[1 5] Together with eclampsia, PE is responsible for 10 15% of the over 500,000 deaths from pregnancy-related disorders globally (99% of these in developing countries).[1,3] In Cuba, PE is among the leading causes of maternal death and there has been an apparent rise in the proportion of maternal mortality related to hypertensive disorders of pregnancy since 2009, but numbers are small: 4 maternal deaths in 2012, a rate of 3.2 per 100,000 live births.[6,7] The precise etiology of PE is unknown.However, it is considered a complex, multfactoral disease, in which individual manifestations depend on the interaction of two or more maternal genes with the fetal genotype and with environmental factors.[8] The main risk factors associated with PE and eclampsia are maternal age (risk is higher at the extremes of age, <18 or >35 years), nulliparity, malnutrition, poverty, low educational level, multiple pregnancy, molar pregnancy, diabetes and lupus erythematosus.[8] Other factors associated with elevated PE risk are s protein deÞ ciency, anticardiolipin antibodies, pre-pregnancy obesity, and genetic factors such as family history (mothers, sisters and daughters of PE patients have greater frequency of the disorder).[6,8] Some studies have identiÞ ed chromosome regions and candidate genes whose variants are related to greater PE susceptibility, but their results have not been replicated consistently across populations.[9,10] The following have been shown to increase PE susceptibility in different settings: [9] polymorphism of the methylenetetrahydrofolate reductase enzyme gene, a ß avoprotein involved in homocysteine remethylation; a common mutation leading to substitution of a glutamine by an arginine at position 506 in the gene encoding for factor V Leiden; angiotensinogen gene polymorphisms; common polymorphisms in the gene encoding for lipoprotein lipase; and the NOS3 gene, located at region 7q36, which encodes for endothelial nitric oxide synthase.
The US National Center for Biotechnology Information genome database includes other genes that may predispose to PE: the TGF-a gene; the alpha-adducin gene, which encodes a protein associated with calmodulin, whose mutations generate changes in blood pressure regulation in rats; the tachykinin receptor 1 gene, related to neurokinin B, a substance implicated in vascular regulation in PE; the gene for annexin 4, a placental anticoagulant protein; adhesion molecule genes; and many others that could be related in some way to PE physiopathology.[8,9] Evidence has accumulated on the respective genetic and environmental contributions to PE predisposition, [8,11,12] but the potential interaction of the two should also be considered.This is fundamental if the goal is proper risk assessment for personalized preventive genetic counseling and more effective prenatal care

O ri gi nal R esearch
Peer R evi ew ed to prevent pregnancy complications.Although there have been Cuban PE risk-factor studies, [13 15] no studies have examined the effect of genetic and environmental components and their interaction on PE risk.This may explain why there are no risk reference tables for PE attributable to genetic predisposition (signaled by family history), environmental risk factors, or their interaction.This problem leads us to ask: Does interaction between a predisposing genome and environmental risk factors contribute to PE risk beyond the effect of the individual factors acting independently?
Hence the hypothesis is put forward that the interaction of a woman s genetic predisposition to PE and adverse environmental factors could increase risk of PE more than if genetic and environmental factors were acting separately.Ours is the Þ rst Cuban study to address this hypothesis.

Study uni verse
This consisted of the 124 pregnant women admitted to the hospital s hypertension and pregnancy service, their records culled from the hospital database.Of these, the 80 patients who met the inclusion criteria below were selected for the study population.

Statistical analysis
Environmental risk factors The Pearson chi-square test for independence and homogeneity and Fisher exact test were used, with statistical signiÞ cance level set at  = 0.05.For factors for which distribution was statistically signiÞ cant between cases and controls, phi () correlation coefÞ cient and odds ratio (OR) were calculated as measures of association strength.The null hypothesis for a difference of proportions of two independent samples was tested, using MICROSTAT statistical software.All non-genome-dependent factors were considered environmental for this analysis.
Genetic factors Familial aggregation was studied for each degree of kinship, using a case-control approach and always excluding the proband (pregnant subject for whom pedigree was constructed).[16] The heritability coefÞ cient (h²) was determined from the correlation between Þ rst-degree (sisters) and second-degree (half sisters) relatives, estimated by the proportion of concordance of the proband with each group.
The following formula was used to calculate h²: h 2 = 4 x (correlation between full siblings minus correlation between half siblings) Since heritability is a proportion, the maximum value is 1; if the value is >0, both genetic and environmental factors are involved in disease occurrence.If h 2 is >0.75, genetic involvement is considerable.[17] Study of genome-environment interaction A casecontrol study was designed to calculate the OR for genome-environment interaction.Analysis of PE family history by kinship degree was considered a proxy for the genome.[17] The three environmental risk factors and three genetic risk factors (kinship degree) with the highest ORs for PE were selected for analysis.Interaction was considered important if the observed OR for the effect of genetic and environmental factors together ORge, (OR gene environment interaction) was greater than expected in both additive (ORg + ORe) and multiplicative (ORg x ORe) models.[17] Ethics Written informed consent was obtained from patients and relatives involved in the study and all information collected was kept conÞ dential.The study was approved by the research ethics committee of the National Medical Genetics Center.

Study of environmental risk factors
The most frequent nongenome dependent factors in both cases and controls were nulliparity, smoking, excessive weight gain, and BMI 25.All these environmental risk factors studied were signiÞ cantly more frequent in cases than in controls, with the exception of nulliparity (Table 2).Of these, case control analysis found Þ ve to be statistically signiÞ cant risk factors for PE, with greatest ORs being alcohol consumption, age >35 years and age <20 years at delivery (Table 3).

Study of genetic factors
Family history of PE was more common in cases than in controls, most frequently in aunts, mothers and sisters-in-law, and least frequently in sisters (  4).
As the proportion of shared genes increased, so did PE probability in the family history, signiÞ cantly more so in cases than in controls.
Overall, higher correlation values were observed for family history than for environmental factors (Tables 3 and 4).The heritability coefÞ cient (h2) was 0.24, indicating involvement of both genetic and environmental factors in PE.

Study of genome-environment interaction
Of the nine interactions, Þ ve were statistically signiÞ cant for both the additive and multiplicative models (Table 5).Having an affected sister and one of the three environmental risk factors with the highest signiÞ cant ORs (alcohol consumption, aged >35 years and aged <20 years at delivery) ampliÞ ed interaction impact on risk.Furthermore, when aged <20 years interacted with genetic susceptibility, the result was always statistically sig-niÞ cant.When a woman with a sister with PE history also used alcohol, PE risk increased by 16 times (ORge = 16.00),compared to the expected OR for an independent effect for either factor (OR for history of sister with PE = 1.61 and OR for alcohol consumption = 4.44; additive model expected OR 6.05, multiplicative 7.44).When a grandmother with PE was identiÞ ed and the pregnant woman was aged <20 years at delivery, the OR for the interaction was 21.07 (greater than the OR of each of these factors separately), and if the pregnant woman was aged <20 years and her husband s sister had PE, then ORge = 14.37 (Table 5).

Analysis of environmental risk factors
In this study, alcohol consumption was the environmental factor associated with greatest PE risk, almost quadruple that of non-drinkers.In Mexico, Morgan-Ortiz found an even stronger association between alcohol use and PE risk (OR 5.77, 95% CI: 1.48 22.53).[18] However, the finding is not seen in all populations.In Thailand, Fang found no association between alcohol consumption and PE.[19] Differences among these studies could be related to heterogeneous distribution of PE prevalence worldwide and with varying effectiveness of health interventions to reduce PE risk.[20,21] Another factor may be failure to quantify amount of alcohol, a limitation of Fang s study as well as our own, in which alcohol use was treated as a nominal dichotomous variable.
It would be worthwhile to conduct further studies with amount consumed as a categorical variable quantiÞ ed in a range from least to greatest values, to more accurately estimate associated risk; and to determine risk resulting from the interaction of this environmental factor, taking into account dose and genetic predisposition assessed by family history.It would also be useful to more accurately specify when alcohol intake took place, whether before or during pregnancy.
Multiple studies have demonstrated an association between age of the pregnant woman (<20 years and >35 years) and greater risk of PE.Authors in Thailand, Iran and Chile observed doubling of PE risk in such cases.[19,22,23] Suárez reported a high incidence of hypertensive disease of pregnancy in Cuban adolescents and women aged >35 years in Santa Clara during the period January 2006 December 2008.[15,24] It is hypothesized that women aged >35 years have a greater frequency of chronic vascular diseases, leading to increased antiangiogenic factors and abnormal placentation, thus increasing susceptibility to PE. [25] It has been posited that abnormal placentation is more frequent in younger patients, consistent with the theory inadequate placentation as the etiological mechanism for PE.[25] We consider age extremes at pregnancy to be modiÞ able risk factors, important for primary health care, since part of the primary prevention activities of the physician, family nurse, genetic counselor, and other professionals is prevention of pregnancy at extreme ages through education and timely preconception counseling.However, a multivariate logistic regression study is recommended to rule out possible confounding effects of variables such as smoking and age, which we did not do in this study.
Current thinking on PE pathogenesis is that it arises because of immune maladaptation between mother and conceptus, which can help explain why nulliparity is a risk factor.[26,27] During the Þ rst pregnancy, paternal antigens in the fetoplacental unit foreign to the host mother activate an immunological mechanism, posited as the trigger for the process leading to vascular damage, the direct cause of PE onset.In turn, immunological tolerance would also develop, which would prevent the disease from developing in subsequent pregnancies, provided the father was the same.[28,29] Another possible explanation suggested for the association between nulliparity and PE risk is that because the nulliparous woman s uterus has not previously been subject to distention of pregnancy, it has increased myometrial tone during the entire pregnancy, which reduces the caliber of the spiral arterioles by compression.This limits blood perfusion in the region, with the consequent possibility of trophoblast hypoxia, a phenomenon that has also been implicated in the physiopathology of PE. [4] These physiopathological underpinnings afÞ rm nulliparity as an important risk factor, even though this study did not obtain this Þ nding.This might be due to the small size of the study group and to the fact that we did not collect information on previous interrupted pregnancies in nulliparous women, both study limitations.It has been shown that a history of spontaneous or therapeutic abortion in primiparous women reduces PE risk.[30,31] We recommend that future research use a larger sample and include detailed inquiry into obstetric history, including previous interrupted pregnancies.
Smoking was statistically signiÞ cantly more frequent in cases than in controls, suggesting a role as a risk factor for PE, as observed by Ioka.[32] However, some studies have described smoking as a protective factor, probably due to increased maternal placental growth factor and increased soluble fms-like tyrosine kinase-1 in maternal blood of women with abnormal uterine artery Doppler Þ ndings.[33] One limitation of our study was that smoking and tobacco use were treated as dichotomous variables; studies with both larger numbers and more detailed history about dose and duration of exposure could be more informative.

Analysis of the genetic component of PE
Women whose Þ rstand second-degree female relatives had PE were more likely to develop it.The increased risk was strongest if the affected relative was the grandmother.Interestingly, the husband s female relatives PE experience also affected risk: a pregnant woman whose sister-in-law had PE had increased risk compared to pregnant women whose sisters-in-law had normal pregnancies.
The familial distribution of PE has been well established and is described by Chesley.[34] It is believed that Þ rst-degree relatives of affected women have two to Þ ve times greater risk of developing PE.In addition, there have been reports of risk of recurrence ranging widely, from 7.5% to 65%.With the development of molecular genetics, evidence has accumulated on the genetic contribution to PE susceptibility.[34 37] A study in Sweden demonstrated the existence of familial clustering in PE, Þ nding ORs of 3.3 and 2.6 for sisters and daughters of a mother with PE, [38] consistent with our results.
Bezerra reported a statistically signiÞ cant increase in PE risk in women whose mother or sister had the condition (p <0.007 and p <0.001 respectively).The association was stronger when both mother and sister had been affected (OR: 3.65).[39] Cruz found that female Þ rst-degree relatives of a woman who has had PE have a four to Þ ve times greater PE risk when they become pregnant.Similarly, second-degree relatives have a two to three times greater risk.[40] Berends also reported evidence of familial clustering in women with a history of PE, in a genetically isolated population.[41] Original Research Chromosome regions associated with PE have been identiÞ ed, but not yet speciÞ c genes.The most studied polymorphisms are: those related to vasoactive genes (M235T of the AT gene, I/D of the ACE gene, E298D of the eNOS gene); mutations of thrombophilic genes (1691G>A of Leiden factor V, 677C>T of the MTHFR gene, 20210G>A of the prothrombin gene); lipid metabolism and oxidative stress genes (Exon3 Tyr3His of the EPHX gene, Exon 6 Asn291Ser of the LPL gene); and genes involved in immune and inß ammatory responses (-308G>A of the TNF gene, -1082G>A of the IL10 gene); and genes involved in immunogenetics, placentation and genomic imprinting.[8,42,43] The additive action and interaction of some of these genes could explain the genetic predisposition for PE in some of the subject families in our study.
Heritability (h 2 ) is the parameter that estimates the relative contribution of genetic factors to the appearance of diseases such as PE.In this study, it was greater than 0 but less than 0.75 (h 2 = 0.24), which means that PE is not due exclusively to environmental factors, but must involve as well a genetic predisposition that is modulated by adverse environmental factors.
It should be noted that blood pressure values in pregnancy are a continuous variable due to the action of genetic and environmental factors or both, but the roles of these factors are still incompletely understood.
An interesting Þ nding in this study is that PE risk increases when the husband has relatives with PE history, even when the woman s own family history is negative.If the husband has a sister or mother with PE history, his wife s likelihood of developing PE increases by factors of 6.71 and 1.99 respectively.Paternal genetic predisposition represented through the fetal genome is one more element in this network of interactions for PE occurrence.[44] There is evidence in the scientiÞ c literature supporting fetal genetic effects on adverse pregnancy outcomes.Fetal genes from both parents are involved in control of placental and fetal tissue growth, and the placenta and membranes play an important role, which fetal genes could inß uence.Additionally, heritability studies estimating the relative portion of population variation in a genetically determined trait suggest that PE is inß uenced in part by fetal genetic factors.[45] Fathers with a family history of PE increase the probability that their pregnant partner will develop it, but some authors report that a change in partners between pregnancies reduces risk of preterm birth in pre-eclamptic women.[46 49] These data suggest that paternal genes expressed in the fetus may contribute to PE.This has prompted studies of maternal fetal inß uence, assessed by deÞ ning the infant as proband, rather than the mother, broadening the scope of investigation beyond speciÞ c maternal inß uences.[46 49] The genome of the partner of the women in our study may present some of the molecular polymorphisms predisposing for PE previously mentioned, inherited from their probably pre-eclamptic mothers, and leaving a genetic imprint on the fetal genome.
Analysis of genome-environment interaction in PE Finally, the Þ ve statistically signiÞ cant interactions that were found dem-onstrate how environmental factors tend to modulate genetic predisposition as reß ected in family history.On a practical level, these Þ ndings could strengthen primary prevention through genetic counseling.Counselors would need to assess whether there is a clear probability of PE in the patient if she has a Þ rst-, second-or third-degree family history of PE (non-modiÞ able risk factor), since they have 50%, 25% and 12.5% genes in common respectively.However, there are modiÞ able environmental factors that could modulate this susceptibility.
In this study, we observed that coexistence of Þ rst-degree family history (such as an affected sister) with alcohol consumption increased PE risk by 16 times.If prevention activities had managed to eliminate alcohol consumption, this risk would have decreased the OR from 16 to 1.61.
The results tend to be similar for the rest of the interactions.It was shown that when environmental risk factors interact with presence of an affected Þ rst-degree relative (family history), probability of PE increased, more than if these factors were acting in isolation.ORs were always greater when a non-genome-dependent predisposing factor interacted with the affected relative.Of these, alcohol consumption was the greatest.
It is interesting to note that there has been relatively little research on gene environment and genome environment interactions in PE.Since it is a multifactoral disease, genetic susceptibility to it will depend on other non-modiÞ able risk factors and modiÞ able ones (such as unhealthy behaviors) that could modify the expression of gene polymorphisms that predispose for PE.
The epigenetic regulation mechanisms described in the biology of the placenta are a focus of attention for researchers investigating environmental factors that modify expression of placental genes, and how these relate to diseases of gestation and the Þ rst years of life.Understanding epigenetic alterations in the placenta can inform diagnosis and prognosis of many diseases such as PE, and could help tailor health promotion and prevention activities. [50]

CONCLUSIONS
Our results, limitations discussed above notwithstanding, con-Þ rm that the risk attributable to the interaction of genetic and environmental factors is greater than the potential risk of these factors acting separately.This study found familial clustering of PE, representing the Þ rst Cuban evidence using genetic epidemiology family pedigree strategy addressing the following questions: Does PE preferentially cluster in families of cases vs. families of controls?Is this familial clustering due to genetic factors?A heritability coefÞ cient of 0.24 demonstrates that both genetic and environmental factors inß uenced PE risk in the women studied.
These Þ ndings conÞ rm the importance of preconception and prenatal care in primary health care, modifying environmental factors that could enhance or trigger polymorphic genes that predispose for PE.To this end, further studies of this type are needed in different regions of Cuba, building on the results presented here.
Finally, this Þ rst-of-its-kind Cuban study is important to maternal and fetal health.It is imperative to expand upon this initial effort

Tabl e 1: Study vari abl es Vari abl e D efi ni ti on
Any use, regardless of number of cigarettes or interruption during pregnancy Alcohol use Yes/No Any use, regardless of dose or frequency Family members with PE Yes, No Mother, sister, aunt, grandmother, half sister, mother-in-law, sister-in-law BMI: body mass index PE: pre-eclampsia in Microsoft Excel 2007, and analyzed using SPSS v. 20.0 and INFOSTAT statistical software.

Table 2
). Familial clustering of PE was observed in relatives of cases compared to those of controls (p <0.05).Among the environmental risk factors studied, alcohol showed the strongest effect on pre-eclampsia risk (OR 3.87, 95% CI 1.64 9.13).Familial pre-eclampsia clustering was observed; risk was increased for both Þ rst-degree (OR 2.43, 95% CI 1.62 3.73) and second-

Table 2 : Distribution of environmental risk factors and family history of pre-eclampsia in cases and controls Risk Factors Cases (n = 80)
(OR 1.89, 95% CI 1.34 2.68) relatives as well as for husband s relatives (OR 2.32, 95% CI 1.40 3.86).Based on OR, family histories conferring greatest PE risks were those with PE grandmothers (7.91), sisters-in-law (6.71) and half sisters (5.93) (Table degree

Table 4 : Case-control analysis of familial PE clustering by degree of kinship
PE: pre-eclampsia

Table 5 : Interactions between most signifi cant environmental and family history PE risk factors
ORg: odds ratio for predisposing genome by family history alone ORe: odds ratio for environmental risk factors alone ORge: odds ratio for gene environment interaction PE: pre-eclampsia * Statistically signiÞ cant interaction

Table 3 : Case-control analysis of hypothesized environmental risk factors for PE
BMI: body mass index PE: pre-eclampsiaOriginal ResearchPeer Reviewed